Fig. 2.

BA compositions in GBs and small intestines from WT and Shp^−/− mice. A: BAs extracted from GBs of experimental animals fed CD or WD were analyzed using an LC–MS. Percent values calculated from their average peak intensities (N; WTC = 4, SHPKOC = 6, WTWD = 5, and SHPKOWD = 5) were plotted into a stacked column graph. B: BAs were extracted from weighed ilea, and their compositions were analyzed. Percent values were calculated from their average peak intensities normalized by weight and were plotted in a stacked column graph (N = 6). C: TDCA, TMC, and TCA values from GBs were plotted with average ± SEM. Two-way ANOVA was performed to obtain statistical values. CA, cholic acid; Gen, genotypes; GCA, glycocholic acid; GLCA, glycolithocholic acid; MCA, muricholic acid; TCA, taurocholic acid; TCDCA, taurochenodeoxycholic acid; TDCA, taurodeoxycholic acid; THCA, taurohyocholic acid; THDCA, taurohyodeoxycholic acid; TLCA, taurolithocholic acid; TMCA, tauromurcholic aicd; TMDCA, tauromurideoxycholic acid; TUDCA, tauroursodeoxycholic acid; UDCA, ursodeoxycholic acid.