Fig. 6.

Reduced endotoxemia and gut permeability in Shp^−/− mice fed WD. A: LPS levels were measured in serum collected from WT and Shp^−/− mice generated by heterozygous mating. Their average values were presented with SEM (n = 6–7). B: Fecal albumin levels in the experimental animals were quantified and plotted as means ± SEM (n = 8–10). C and D: quantitative PCR analysis was performed to quantify mRNA levels of indicated genes involved gut permeability and fat absorption in their small intestines from separately housed (C) and cohoused mice (D). n = 5–8. Two-way ANOVA and Student's t-test were performed to evaluate statistical significance. #comparison with CD, ∗comparison with WT counterparts. Three symbols represent P < 0.005, two symbols P < 0.01, and one symbol P < 0.05.