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. 2023 Dec 5;14:8051. doi: 10.1038/s41467-023-43904-w

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a Delivery of ABE8e-Cas9 RNP targeting B2M locus using S10 and S315 peptides in human airway epithelial cells cultured at the air liquid interface. Editing efficiency was assessed using HTS. Top panel shows the target DNA strands and PAM sites (blue font). Yellow highlight denotes the predicted 4–8 nt ABE editing window (numbered). Target A residue is in red font. Average frequency of desired product for human B2M locus with the indicated shuttle peptides. b Graphic representation of desired base editing efficiency at human B2M locus using S10 (blue column) and S315 (red column) peptides. Individual closed circles represent data from 4 technical replicates, and the data is plotted as mean ± SEM. Statistics by unpaired two-tailed t test, ***P = 0.0005. c Delivery of ABE8e-Cas9 RNP targeting CCR5 locus using S10 and S315 peptides in rhesus tracheal epithelial cells cultured at the air liquid interface. Editing efficiency quantified using HTS. Top panel shows the target DNA strands and PAM sites (blue text). Yellow highlight denotes the predicted 4–8 nt ABE editing window (numbered). Target A residue highlighted in red text. Target A residue values are inlarge font. Average frequency of desired product for rhesus CCR5 locus with the indicated shuttle peptides. d Graphic representation of desired base editing efficiency at rhesus CCR5 locus using S10 (blue) and S315 peptides (red). Individual closed circles represent data from 4 technical replicates, and the data are plotted as mean ± SEM. Statistics by unpaired two-tailed t test, ***P = 0.0006. Source data are provided as a Source Data File.