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. 2023 Dec 5;14:8051. doi: 10.1038/s41467-023-43904-w

Fig. 3. Intratracheal in vivo delivery of DRI-NLS-Cy5 with S10 shuttle peptide results in widespread distribution and cell-type specific delivery in rhesus monkey airways.

Fig. 3

a Representative epifluorescence microscopy images of the rhesus lung tissue sections demonstrating the DRI-NLS-Cy5 localization in the surface epithelial cells of airways of various sizes, ranging from trachea to small airways and alveolar regions (Fig. 3a, top panel). Control received DRI-NLS-Cy5 alone (Fig. 3a, bottom panel). Scale bar is 100 µm. b Confocal microscopy images documenting localization of DRI-NLS-Cy5 fluorescence (white) in ciliated cells (*, AcTub - red), secretory cells (#, SCGB1A1 – green, top panel), and very rarely to CK5+ basal cells (*, CK5 – green, middle panel), where DAPI is pseudo-colored blue. In the alveolar regions (Fig. 3b, lower panel), the DRI-NLS-Cy5 signal (white) localized to the nuclei of the surfactant protein C producing alveolar type II cells (#, SP-C - red) and alveolar macrophages (*, CD68 - green). Scale bar is 20 µm. For (a) and (b), the staining and microscopy was performed at least two times on tissue sections from different regions of the lungs and yielded similar results.