Table 3.

In this study, we analyzed the expression of the iNOS gene in mice and the kDNA of Leishmania parasites. This analysis included specific primer sequences, along with corresponding annealing temperatures and the size of the resulting amplification product (amplicon size). GAPDH was used as a reference in these investigations.

Gene	Primer sequence	Annealing temp (°C)	Amplicon size (bp)
iNOS	F_5-CCCTTCCGAAGTTTCTGGCAGCAGC-3 R_5-GGCTGTCAGAGCCTCGTGGCTTTGG-3	56	101
GAPDH	F_5-CAAGGCTGTGGGCAAGGTCA-3 R_5-AGGTGGAAGAGTGGGAGTTGCTG-3	56	242
Leishmania kDNA	F_5-CTTTTCTGGTCCTCCGGGTAGG-3 R_5-CCACCCGGCCCTATTTTACACCAA-3	56	140