Table 2.

Thermal kinetic parameters of the purified A. fumigatus Amidase

Temp (°C)	T1/2 (h)		Kr (min) × 10–3
4	250	0.002
20	75.7	0.15
30	29.93	0.245
40	16.30	0.499
50	13.37	0.832

^*Half-life time (T_1/2) was expressed by the time at which the enzyme retains 50% of its initial activity by preheating without substrate at each temperature degree

^**Thermal denaturation rate (K_r) was expressed by the logarithmic decreasing of enzyme activity with the time at each temperature

(ln (At/A0) =−Kr, where A0 and At are the specific activity of Asnase at zero and t time

It is described by the first-order kinetic model