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. 2023 Dec 5;14:8054. doi: 10.1038/s41467-023-43623-2

Fig. 2. Skin of subterranean species contains higher levels of HA.

Fig. 2

a Cryosections of skin of different species were stained with hyaluronan binding protein (HABP, red) and Hoechst (blue). Representative immunofluorescence images are shown at 20 × magnification (left). Scale bar, 50 μm. Quantification of HABP fluorescence intensity (right panel) was performed on the similar regions of each sample as indicated by the squares with dashed outline (left panel). For each sample, cumulative intensities were normalized to the area of selected regions to control the volume. Data are mean ± SD of three independent areas. *P < 0.05, **P < 0.01, and ***P < 0.001 comparing subterranean species with their aboveground controls by unpaired two-sided t test. b RT-qPCR showing expression levels of HAS2 and HYAL2 genes in the skin comparing subterranean species with their aboveground controls. Experiments were performed using three biological replicates except mouse, EM and SNM, which had two biological replicates. Red bars indicate subterranean species. Data are mean ± SD. *P < 0.05, **P < 0.01 by unpaired two-sided t test. Source data are provided as a Source Data file.