TABLE 2.
Viabilities of single-strand exonuclease-deficient strains
Strain | Relevant genotype | CFU/ml (109)a | No. of observed cells/ml (109)b | Viability (CFU/cell counted) |
---|---|---|---|---|
JC11450 | xonA+ xseA+ recJ+ F− | 2.6 ± 0.13 | 2.9 ± 0.26 | 0.9 |
SMR3488 | JC11450 ΔxonA ΔxseA recJ | 2.7 ± 0.14 | 2.1 ± 0.28 | 1.3 |
SMR3524 | JC11450 mutL | 2.9 ± 0.11 | 2.4 ± 0.75 | 1.2 |
FC40 | xonA+ xseA+ recJ+ F′ | 2.9 ± 0.09 | 2.8 ± 0.31 | 1.0 |
SMR3481 | FC40 ΔxonA ΔxseA recJ | 2.6 ± 0.18 | 2.3 ± 0.23 | 1.1 |
SMR3404 | FC40 mutL | 2.3 ± 0.07 | 1.2 ± 0.17 | 1.9 |
Mean number of CFU per milliliter ± one standard error of the mean for five independent cultures grown to saturation in Luria-Bertani-Herskowitz (LBH) broth (see, e.g., reference 36).
Mean number of cells per milliliter ± one standard error of the mean in the same five saturated cultures determined with a Petroff-Hausser counter.