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. 2023 Nov 27;19:101728. doi: 10.1016/j.bonr.2023.101728

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© 2023 The Authors. Published by Elsevier Inc.

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Fig. 3 — Immunofluorescence analysis of human chondrocytes in control (CTR) and in a patient carrying COL2A1 (p.Gly444Ser) variant. (A) Immunostaining of collagen II (green) in cells at days 0, 21 and 28 of chondrogenic differentiation and high magnification on the right (high magnification). Statistical analysis is reported in the graphs on the right. (B) Double immunofluorescence analysis of collagen II (green), and golgin 97 (red) in human chondrocytes at day 28 of differentiation. High magnification in the panels below (high magnification). Statistical analysis is reported in the graph below. (C) Immunostaining of fibronectin (green) in human chondrocytes at day 28 of differentiation. 4,6-diamidino-2-phenylindole (DAPI, blue) was used to counterstained cell nuclei. Scale bars, 10 μm. Three biological replicates were used in each experiment and statistically significant differences (p < 0.001) between values are indicated.