Table 1.

True diffusion coefficients after accounting for localization uncertainty

PAmCherry construct	Strain	${\mathrm{D}}_{\text{true}}^{\text{slow}}($μm2/s)	${\mathrm{D}}_{\text{true}}^{\text{fast}}$ (μm2/s)
BamA	MSC18	0.0002 (6)	−0.002 (4)
OmpLA	MC4100	0.001 (2)	0.021 (1)
BamA barrel	MC4100	0.001 (1)	0.018 (3)
FL-BamA	JCM166	0.0001 (5)	0.002 (2)
ΔP1 BamA	JCM166	0.0013 (6)	0.007 (4)
ΔP12 BamA	JCM166	0.003 (1)	0.006 (3)
SecE	MC4100	0.001 (2)	0.005 (1)
PgpB	MC4100	0.0028 (8)	0.039 (4)
SecE	JCM166/FL-BamA	0.001 (1)	0.0052 (5)
	JCM166/ΔP1 BamA	0.0020 (8)	0.0163 (9)
	JCM166/ΔP12 BamA	0.0019 (9)	0.0167 (7)
PgpB	JCM166/FL-BamA	0.0022 (6)	0.0351 (4)
	JCM166/ΔP1 BamA	0.0029 (7)	0.0371 (8)
	JCM166/ΔP12 BamA	0.0026 (9)	0.0363 (6)

These values were obtained by calculating the y intercept when plotting D_app vs. lag time. The number in parentheses represents uncertainty in the least significant digit as given by the SE of the mean of these values calculated from experimental replicates. Strains denoted with a “/” are composed of the strain on the left side transformed with a plasmid containing the construct on the right side (without a fluorescent tag).