TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Laboratory stock no. | Relevant characteristics | Reference or source |
|---|---|---|---|
| E. coli K-12 strain SØ874 | P4052 | Δ(sbcB-dcd) | 7 |
| P5470 | P4052 carrying plasmids pPR1857 and pGP1-2 | This study | |
| P5282 | P4052 carrying plasmids pGEM-T and pGP1-2 | This study | |
| Plasmids | |||
| pGEM-T | Cloning vector; Apr; replicon, pMB1 | Promega | |
| pGP1-2 | Bacteriophage T7 RNA polymerase gene cloned into pACYC177; replicon, p15A | 10 | |
| pPR1857 | K-12 CA DNA from positions 8621 to 10722 cloned into pGEM-T | This study |