TABLE 1.
Bacterial strains and plasmids used
| Strain or plasmid | Lab no. | Characteristics | Reference or source |
|---|---|---|---|
| E. coli K-12 DH10b | P5135 | araD139 Δ(ara-leu7697) galU galK mcrA Δ(mrr-hsdRMS-mcrBC) rpsL deoR, φ80dlacZDM15 endA1 nupG recA1 | 9 |
| S. enterica LT2 group B | |||
| LV386 | P9350 | wzy::aph (kanamycin, spectinomycin, and streptomycin resistant) | 4 |
| SL1654 | P9003 | hsdL6 trpC2 fliB flaA66 rpsL120 xylT404 ilvE452 metE551 metA22 hsdSA29 (streptomycin resistant) | 31 |
| P9487a | P9487 | SL1654 carrying wzy::aph (kanamycin and streptomycin resistant) | This study |
| S. enterica group D3 | |||
| 877 K | M840 | S. II sv. 1,9,12,46,27:C:Z39 (formerly called serovar Zuerich) | Pasteur Institute |
| 2764/79 | M1168 | S. II sv. 1,9,12,46,27:Z10:e,n,x | Pasteur Institute |
| 8316/94 | M1169 | S. II sv. 1,9,12,46,27:a:Z6 | Pasteur Institute |
| 149/66 | M1170 | S. II sv. 1,9,12,46,27:y:Z39 | Pasteur Institute |
| 3334/81 | M1171 | S. II sv. 1,9,12,46,27:Z10:1,5 | Pasteur Institute |
| Plasmids | |||
| pPR1739 | 3.2-kb PCR product from group D3 carrying wzy gene cloned into pGEM-T vector (ampicillin resistance) | This study | |
| pPR1820 | wzyD3 and wbaOE1 genes cloned into vector pPR637 (13) (spectinomycin and streptomycin resistance) | This study | |
| pPR618 | tyv and prt genes cloned into pUC18 (ampicillin resistance) | 37 |
a
Made as follows: chromosomal DNA of LV386 was sheared by vortexing and transformed into strain SL1654 by electroporation; a kanamycin-resistant recombinant was selected.