| Reagent/Resource | Reference or source | Identifier or catalogue number |
|---|---|---|
| Experimental models | ||
| HAEC (H. sapiens) | Gibco | C0065C |
| Antibodies | ||
| Anti‐ICAM‐1 monoclonal antibody, mouse (1:200 IF) | Cell Signalling | 62133 |
| Anti‐VE‐cadherin monoclonal antibody, rabbit (1:200 IF) | Cell Signalling | 2500 |
| Goat anti‐Rabbit IgG (H+L) Cross‐Adsorbed secondary antibody, HRP | ThermoFisher Scientific | G‐21234 |
| Anti‐mouse 555 goat secondary antibody (1:500 IF) | ThermoFisher Scientific | A21422 |
| Anti‐rabbit 647 goat secondary antibody (1:500 IF) | ThermoFisher Scientific | A21244 |
| Anti‐HIF1α monoclonal antibody, rabbit (1:500 WB) | Cell Signalling | 36169 |
| Anti‐GAPDH polyclonal antibody, rabbit (1:2,000 WB) | Abcam | ab22555 |
| Oligonucleotides and other sequence‐based reagents | ||
| siRNA | Dharmacon (Horizon Discovery) | |
| ALDO A primers |
ThermoFisher Scientific This study |
|
| LDHA primers |
ThermoFisher Scientific This study |
|
| CXCR4 primers |
ThermoFisher Scientific This study |
|
| NOS3 primers |
ThermoFisher Scientific This study |
|
| Cyclophilin primers |
ThermoFisher Scientific This study |
|
| Chemicals, Enzymes and other reagents | ||
| EGM‐2 Endothelial Cell Growth Medium‐2 Bullet Kit | Lonza | CC‐3162 |
| Lipofectamine RNAiMAX Transfection Reagent | ThermoFisher Scientific | 13778150 |
| Opti‐MEM Reduced Serum Medium | Gibco | 31985070 |
| Hoechst 33342 | ThermoFisher Scientific | H3570 |
| DMSO | Sigma‐Aldrich | D8418 |
| IL‐1β | Peprotech | 200‐01B |
| OXPAC | Hycult Biotech | HC4036 |
| CM‐H2DCFDA | ThermoFisher Scientific | C6827 |
| Forbol 12‐miristato‐13‐acetato (PMA) | Sigma‐Aldrich | P8139 |
| H2O2 | Sigma‐Aldrich | H1009 |
| Atorvastatin | Sigma‐Aldrich | SML3030 |
| Mitosox | ThermoFisher Scientific | M36008 |
| Paraformaldehyde (PFA) | Sigma‐Aldrich | 158127 |
| Triton X‐100 | Sigma‐Aldrich | T8787 |
| Bovine Serum Albumin (BSA) | Sigma‐Aldrich | A7906 |
| DAPI | ThermoFisher Scientific | D3571 |
| RIPA lysis buffer | Millipore | 20‐188 |
| Protease Inhibitor Cocktail | Sigma‐Aldrich | P8340 |
| Phosphatase Inhibitor Cocktail 2 | Sigma‐Aldrich | P5726 |
| Phosphatase Inhibitor Cocktail 3 | Sigma‐Aldrich | P0044 |
| PVDF transfer membranes | Millipore | IPVH00010 |
| TRIzol reagent | ThermoFisher Scientific | 15596026 |
| SuperScript IV Reverse Transcriptase | ThermoFisher Scientific | 18090010 |
| Software | ||
| Bowtie2 | Langmead and Salzberg (2012) | |
| MACS2 tool | Kim and Dekker (2018) | |
| rtracklayer and GenomicRanges | Lawrence et al (2013) and Lawrence et al (2009) | |
| ChIPSeeker R package | Yu et al (2015) | |
| ChIPpeakAnno R package | Zhu et al (2010) | |
| rGADEM and MotIV | R packages | |
| HTSeq2 | Putri et al (2022) | |
| DESeq2 R package | Love et al (2014) | |
| TRRUST database v.2 | Han et al (2018) | |
| R package Seurat v3.2.292 | Hao et al (2021) | |
| ClusterProfiler package | Yu et al (2012) | |
| PageRank | http://igraph.org | |
| OpenTargets | Ochoa et al (2021) | |
| ChEMBL | Willighagen et al (2013) | |
| CellProfiler v4.2.5 |
https://cellprofiler.org/releases Carpenter et al (2006) |
|
| CellProfiler plugins |
https://arxiv.org/abs/2306.01915 preprint: Tromans‐Coia et al (2023) |
|
| ImageJ |
https://imagej.nih.gov/ij/download.html Schneider et al (2012) |
|
| Other | ||
| LIVE/DEAD Viability/Cytotoxicity Kit | ThermoFisher Scientific | L3224 |
| Pierce BCA Protein Assay Kit | ThermoFisher Scientific | 23225 |
| QuantiTect SYBR green PCR kit | Qiagen | 204143 |
| EVOS M7000 image system | ThermoFisher Scientific | AMF7000 |
| ImageQuant LAS 4000 mini | GE Healthcare | |
| QuantStudio 12 K Flex system | ThermoFisher Scientific | |
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