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Polymorphisms that remain stably mutated in the sensitive and methotrexate-resistant Saos-2 cell lines.
| Cell line | Identified variants | |
|---|---|---|
| ABCB1 rs2032582 DNA/RNA | MTHFD1 rs2236225 DNA/RNA | |
| Saos-2 | CA/CA | AA/AA |
| Saos-2/MTX30 | CA/CA | AA/AA |
| Saos-2/MTX100 | CA/CA | AA/AA |
| Saos-2/MTX300 | CA/CA | AA/AA |
| Saos-2/MTX1µg | CA/CA | AA/AA |
| Amino acid change due to a variant | ||
| Saos-2 | Ser-Ala | Arg-Gln |
| Saos-2/MTX30 | Ser-Ala | Arg-Gln |
| Saos-2/MTX100 | Ser-Ala | Arg-Gln |
| Saos-2/MTX300 | Ser-Ala | Arg-Gln |
| Saos-2/MTX1µg | Ser-Ala | Arg-Gln |
