Fig. 3.

In vivo NIR-II imaging and biodistribution of SeCF₃-IRD800 for subcutaneous tumor-bearing mice. (a) In vivo imaging of SeCF₃-IRD800 and ICG. The tumor fluorescence signal acquisition area is shown in the white dotted line box on the right side of mice. (b ∼ c) Quantitative analysis of fluorescence intensity and TBR over 48 h. The fluorescence intensity of tumors increased gradually after injection of SeCF₃-IRD800 and reached the highest fluorescence intensity and the best TBR at 8 h (7658.41 ± 933.34, 5.20 ± 1.04, respectively) (Red line). ICG accumulates in the liver and the tumor has no fluorescence signal. (Blue line) (d) Comparison of liver fluorescence signals between SeCF₃-IRD800 and ICG imaging (SeCF₃-IRD800: Red line. ICG: Blue line). Imaging filter:1000 nmLP. Exposure time:300 ms. Excitation wavelength:808 nm. Laser power density: 21.10 mW/cm². Imaging field of view: 10 cm. The type of camera sensor: InGaAs.