FIG. 2.

Detection of SH subunits by immunoblotting. Soluble extracts (20 μg of protein) were applied to lanes 1 and 3 to 8 and separated on a sodium dodecyl sulfate–12.5% polyacrylamide gel. Lanes: 1, HF424; 2, 0.5 μg of purified SH; 3, H16; 4, HF424(pGE15); 5, HF424(pGE346 [ΔhoxFU]); 6, HF444 (ΔhoxFUY); 7, HF424(pGE371 [ΔhoxYH]); 8, HF424(pGE370 [ΔhoxUYH]). The blot was processed with a mixture of antibodies raised against the four SH subunits. The positions of the molecular mass standards are given on the left.