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Analysis of HoxH processing: Western blot analysis of soluble extracts with antibodies against HoxH. A total of 20 μg of protein was applied to each lane of a sodium dodecyl sulfate–12.5% polyacrylamide gel. Lanes: 1, H16; 2, HF424(pGE15); 3, HF424(pGE346 [ΔhoxFU]); 4, HF444 (ΔhoxFUY); 5, HF14 (hoxW14) (see text); 6, HF14(pGE348 [hoxW]); 7, HF376 (ΔhoxW).
