FIG. 4.

Effect of deletions in the SH genes on ⁶³Ni incorporation into HoxH: analyses of soluble extracts separated on native polyacrylamide gels (4 to 15%). Cells were grown in the presence of 150 nM ⁶³NiCl₂. SH-specific bands are marked by arrows. (A) Western blotting with antibodies against HoxH. A total of 50 μg of protein each was applied to each lane. Lanes: 1, HF424; 2, H16; 3, HF424(pGE15); 4, HF424(pGE346 [ΔhoxFU]); 5, HF444 (ΔhoxFUY). (B) Corresponding autoradiogram. A total of 100 μg of protein was applied to each lane.