TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Relevant characteristicsa | Source(s) or reference |
|---|---|---|
| Strains | ||
| A. eutrophus | ||
| H16 | SH+ MBH+ | DSM 428, ATCC 17699 |
| HF14 | SH− MBH+ | 37 |
| HF376 | SH− MBH+ ΔhoxW | 43 |
| HF387 | SH− MBH+ ΔhoxFUYHW | 28 |
| HF402 | SH− MBH+ ΔhoxFUY | This study |
| HF424 | SH− MBH− ΔhoxFUYHW ΔhoxG | This study |
| HF444 | SH− MBH− ΔhoxFUY ΔhoxG | This study |
| E. coli S17-1 | Tra+recA pro thi hsdR chr::RP4-2 | 42 |
| Plasmids | ||
| pBluescript KS+ | AprlacZ′ T7; φ10 promoter, f1 ori | Stratagene, Cloning Systems |
| pTZ18R | AprlacZ′; f1 ori | 31 |
| pVK101 | Kmr Tcr; RP4 ori | 22 |
| pLO1 | KmrsacB; RP4 oriT, ColE1 ori | 24 |
| pCH241 | 4.6-kb HindIII-KpnI fragment of pGE15 in pTZ18R | C. Böcker and B. Friedrich |
| pCH291 | 2.9-kb HindIII-BamHI fragment of pGE15 in pTZ18R | A. Tran-Betcke and B. Friedrich |
| pCH424 | 2.2-kb SalI-SmaI fragment of pCH423 in pLO1; ΔhoxG | 7 |
| pCH455 | 15.0-kb HindIII fragment of pGE15 in pKS+ | This study |
| pCH494 | Derivative of pCH291 deleted for a 2,390-bp XhoI fragment (deletion of hoxFU) | This study |
| pCH549 | Derivative of pCH241 deleted for a 2,230-bp SacII fragment (deletion of hoxUYH) | This study |
| pCH550 | Derivative of pCH241 deleted for a 1,219-bp EcoRI fragment (deletion of hoxYH) | This study |
| pCH551 | Derivative of pCH455 containing a 2.2-kb AatII-KpnI fragment of pCH549 | This study |
| pCH552 | Derivative of pCH455 containing a 1.2-kb AatII-KpnI fragment of pCH550 | This study |
| pCH568 | Derivative of pCH241 deleted for a 2,650-bp NruI fragment (deletion of hoxFUY) | This study |
| pCH569 | 800-bp HindIII-BstEII fragment of pCH568, filled in with Klenow polymerase, ligated in PmeI site of pLO1 | This study |
| pGE15 | 15.0-kb HindIII fragment of pHG1 in pVK101 | 45 |
| pGE346 | Derivative of pGE15 containing a 0.8-kb HindIII-BamHI fragment of pCH494 (ΔhoxFU) | This study |
| pGE347 | Derivative of pGE15 deleted for a 921-bp PstI fragment (ΔhoxH) | 43 |
| pGE348 | 0.7-kb fragment (hoxW) in pGE151 | 43 |
| pGE370 | 12.2-kb HindIII fragment of pCH551 in pVK101 (ΔhoxUYH) | This study |
| pGE371 | 13.2-kb HindIII fragment of pCH552 in pVK101 (ΔhoxYH) | This study |
a
SH+, SH positive; SH−, SH negative; MBH+, MBH positive; MBH−, MBH negative.