Table 1. CAP Sequences, Abbreviations, and Kinetic Dataa.
| CAP sequence | abbreviation | apparent Kd (μM) | Hill slope (h) | CAP–siRNA particle cluster radius (nm) |
|---|---|---|---|---|
| cyclic-Ac-C[FKFE]2CG-NH2 | [FKFE]2 | 29.0 ± 2.8 | 2.8 ± 0.5 | 188 ± 11 |
| cyclic-Ac-C[FK]4CG-NH2 | [FK]4 | 11.1 ± 0.2 | 16.4 ± 2.3 | 247 ± 35 |
| cyclic-Ac-C[FR]4CG-NH2 | [FR]4 | 14.0 ± 1.5 | 1.6 ± 0.3 | 212 ± 25 |
| cyclic-Ac-C[LR]4CG-NH2 | [LR]4 | 8.6 ± 0.2 | 4.9 ± 0.7 | 209 ± 25 |
| cyclic-Ac-C[YR]4CG-NH2 | [YR]4 | 3.1 ± 0.4 | 1.3 ± 0.1 | 135 ± 18 |
| cyclic-Ac-C[WR]4CG-NH2 | [WR]4 | 2.8 ± 0.3 | 2.1 ± 0.1 | 202 ± 20 |
| cyclic-Ac-C[WK]4CG-NH2 | [WK]4 | 0.87 ± 0.05 | 2.8 ± 0.5 | 135 ± 20 |
| cyclic-Ac-C[WH]4CG-NH2 | [WH]4 | 26.7 ± 3.3 | 2.2 ± 0.5 | 210 ± 30 |
| cyclic-Ac-C[WS]4CG-NH2 | [WS]4 | 26.6 ± 3.8 | 2.0 ± 0.2 | 291 ± 33 |
| Ac-(WR)4G-NH2 | (WR)4G | 30.0 ± 1.1 | 2.7 ± 0.2 | 189 ± 9 |
a
Slot blot filtration assays were utilized to obtain apparent dissociation constants (Kd) and Hill slope values (h). Values are reported as the average of at least three measurements, with the error reported as the standard deviation. Radii of CAP–siRNA nanoparticles were measured via DLS and are reported as the average of at least three measurements, with the error reported as the standard deviation.