Fig. 4.
Bouin’s-fixed Amblyomma americanum salivary gland, capturing Cytauxzoon felis-infected acini using H&E corresponding with two RNAscope ISH probes (Cf-18SrRNA and Cf-GAPDH) in serial sections (arrows, 5–8). Locations on the slide of the demonstrated SG sections are indicated in the top left diagram. There is moderate non-specific background staining mainly along the salivary duct linings (arrowheads). 20X (H&E: Hemotoxylin and eosin; ISH: in situ hybridization).