TABLE 2.
Relationship between growth rate and NEM sensitivity
| Strain | Phenotypea | Growth conditionb | Avg growth rate (h−1) ± SD | Avg T90 (min) ± SDc |
|---|---|---|---|---|
| MSD462 | Clp+ | −CAS | 0.5 ± 0 | 43.5 ± 12 (6) |
| MJF385 | Clp− | −CAS | 0.5 ± 0 | 82.7 ± 1.7 (3) |
| MSD462 | Clp+ | +CAS | 0.8 ± 0 | 33.8 ± 9.4 (12) |
| MJF402 | Clp+ | +CAS | 0.8 ± 0 | 9.3 ± 3.4 (3) |
| MJF385 | Clp− | +CAS | 0.7 ± 0.1 | 60.5 ± 8.8 (4) |
| FRAG1 | Clp+ | −CAS | 0.7 ± 0 | 27.7 ± 6.4 (3) |
| MSD462 | Clp+ | +CAS + bases | 1.1 ± 0 | 6.3 ± 1.2 (5) |
| Frag1 | Clp+ | +CAS | 1.1 ± 0.1 | 6.6 ± 3 (12) |
a
All cells were RpoS+ except MJF402 (MSD462, rpoS::Tn10).
b
Exponential-phase cultures were grown in M9 medium with the defined supplements. CAS and bases (adenine, thymine, cytosine, guanine, and uracil) were added to 0.4% (wt/vol) and 50 μg/ml, respectively.
c
NEM viability experiments were conducted exactly as described in Materials and Methods. T90 is the time taken for 90% of the cells to die upon exposure to 0.3 mM NEM. The data are average values of the number of experiments given in parentheses.