Fig. 3.

CircGPRC5A promotes CRC cell invasion, migration, and proliferation in vitro. A Schematic illustration of the siRNA targeted at the back-splice junctions of circGPRC5A. B–C After transfecting HCT8 and HT29 cells with siRNA or siNC, circGPRC5A expressions were assessed using qRT-PCR. D–H Colony formation and CCK-8 assays were used to measure proliferation after siRNA or siNC transfection into HCT8 and HT29 cells. I–K EdU assay (scalebar: 100 µm) was used to determine whether HCT8 and HT29 cells could proliferate after circGPRC5A silencing. L–N Transwell assays were performed to assess HCT8 and HT29 cell migration and invasion abilities after circGPRC5A silencing. O–Q Following circGPRC5A silencing, wound healing assays were carried out to measure migration and invasion of HCT8 and HT29 cells. Based on data from three independent experiments, values are presented as the mean ± SD. *P < 0.05; **P < 0.01; ***P < 0.001