TABLE 7.
Levels of FtsZ84 and NusA proteins in temperature-sensitive and temperature-resistant strains
| Strain/plasmid | Genotype/plasmid | Normalized relativea amt of FtsZ84
|
|
|---|---|---|---|
| Avg (SD) | Ratio | ||
| BSP610 | ftsZ84 | 0.23 (0.02) | 1 |
| BSP623 | ftsZ84 glnF | 0.92 (0.17) | 3.9 |
| BSP610/pGB2 | ftsZ84/vector | 0.35 (0.02) | 1.5 |
| BSP610/pHM675 | ftsZ84/relA′ | 0.78 (0.16) | 3.4 |
a
Levels of the FtsZ84 and NusA proteins relative to the total protein level were estimated by quantitative immunoblot analysis as described in the text. Raw FtsZ84 values were normalized by division by the respective NusA values and averaged among four appropriately weighted measurements per strain. The average relative amount of NusA among 16 measurements was 0.23, and the standard deviation was 0.03.