Silver-stained SDS-polyacrylamide gel showing purified 4-carboxymuconolactone decarboxylase:3-oxoadipate enol-lactone hydrolase from R. opacus 1CP in lane 2. Lane 1, Pharmacia low-molecular-weight markers: bovine α-lactalbumin (14.4 kDa), soybean trypsin inhibitor (20.1 kDa), bovine carbonic anhydrase (30 kDa), chicken egg white ovalbumin (43 kDa), bovine serum albumin (67 kDa), and rabbit phosphorylase b (94 kDa).