Restriction map of the inserts of pRER3 and pRER4, together carrying 5,475 bp of R. opacus 1CP DNA in the multiple cloning site of pBluescript II KS (+). Arrows indicate the lengths and orientations of open reading frames. Overlapping open reading frames (between pcaH and pcaG, 1 bp; between pcaB, pcaL, and pcaR, 4 bp) are indicated by blunt arrows.