FIG. 6.

Sequence alignment of the 4-carboxymuconolactone decarboxylase:3-oxoadipate enol-lactone hydrolase PcaL of R. opacus 1CP to the 4-carboxymuconolactone decarboxylases PcaC of A. calcoaceticus (M33798 [24]) and B. japonicum (Y10223 [3]), to the N terminus (term.) of PcaC from P. putida (73), and to the 3-oxoadipate enol-lactone hydrolases PcaD of B. japonicum (Y10223 [3]) and CatD and PcaD of A. calcoaceticus (L05770 [25] and M76991 [63]). Positions identical in all compared sequences are highlighted. Possible catalytic triad residues (25, 46) of the hydrolases as well as glycine residues allowing formation of the “nucleophile elbow” (46) are indicated by arrows. Numbers above the sequences refer to positions in the alignment, not in a single sequence.