TABLE 3.
Sequences of N termini, tryptic peptides, and deduced primers
| Protein or peptide | Amino acid sequencea | Deduced primer sequence |
|---|---|---|
| 3-Oxoadipate enol-lactone hydrolase | ||
| N terminus | XXVALA(H)EISGPRNGAAXAPVVVLLGXL | GGYCCSCGNAACGGYGCSGC |
| ELH52.80 | GHGESPAPDGPYSVVR | GTASGGRCCGTCSGGSGCSGG |
| ELH70.65 | WFSEGFAAK | CTTSGCSGCGAARCCYTCSG |
| ELH71.92 | SMWDPQIAALSDE | |
| Protocatechuate 3,4-dioxygenase | ||
| N-terminus α-subunit | MIDTQNP | |
| N-terminus β-subunit | MLHLPAH |
a
Segments used for the design of oligonucleotides for cloning are underlined. X, amino acid that could not be identified.