FIG. 5.

Sequence alignment of the presumed ClcR of R. opacus to known LysR-type regulatory proteins that govern operons for catechol catabolism (CatM and CatR) or chlorocatechol catabolism (ClcR, TfdR, TcbR, and TfdT). Numbers above the sequences refer to positions in the alignment, not in single sequences. Amino acids in positions in which at least seven of the eight sequences are identical are highlighted. Accession numbers and references for the published sequences: TfdR of pJP4, M98445 (46); ClcR of pAC27, L06464 (8); TcbR, M57629 (70); TfdT, U16782 (40); CatM, M76991 (55); CatR, U12557 (35). The amino acid sequence of TfdR from pEST4011 was obtained by translating the DNA sequence after generating a frameshift by insertion of one nucleotide behind position 726 of the submitted sequence (U32188 [38]).