TABLE 1.
Plasmids used in this study
| Plasmid | Relevant characteristicsa | Source (reference) |
|---|---|---|
| pBluescript II KS (+) | Phagemid derived from pUC19; Plac lacZ′ Apr; f1(+) origin; ColE1 origin | Stratagene (2) |
| pRERD1 | Apr; ca. 220-bp PCR product obtained with dioxygenase-derived primers in EcoRV site of pBluescript II KS (+) | This study |
| pRERC1 | Apr; ca. 60-bp PCR product obtained with cycloisomerase-derived primers in EcoRV site of pBluescript II KS (+) | This study |
| pRER5 | Apr; ca. 2.9-kb PstI fragment of R. opacus 1CP DNA in pBluescript II KS (+) | This study |
| pRER6 | Apr; ca. 10-kb HindIII fragment of R. opacus 1CP DNA in pBluescript II KS (+) | This study |
| pRER61 | Apr; ca. 2.4-kb SacII fragment of pRER6 in pBluescript II KS (+) | This study |
| pRER7 | Apr; ca. 3.7-kb EcoRI fragment of R. opacus 1CP DNA in pBluescript II KS (+) | This study |
a
Apr, plasmid carries information for ampicillin resistance.