TABLE 2.
Sequences of N termini, tryptic peptides, and deduced primers
| Protein or peptide | Amino acid sequencea | Deduced primer sequence |
|---|---|---|
| Chlorocatechol 1,2-dioxygenase | ||
| CCD67.8 | VVPAEDG(V)(I)(P)F(H)SIR | |
| CCD72.0 | GNWT(D)(L)AIQGPF | AASGGBCCYTGKATNGC |
| CCD82.6 | VIELFDEF(T)DL(R) | GTSATCGAGYTNTTYGAYGA |
| Chloromuconate cycloisomerase | ||
| N terminusb | PNLTVSGVRTTIVDLPIL(R)P | ACSACSATCGTSGAYYTNCC |
| CMCI65.74 | ELEAIGLSAVPVEEQR | |
| CMCI76.33 | QIIDEYLAPALIGSDVEDLAGTR | |
| CMCI79.76 | VEVVT(D)AGFVGLGEGV | |
| CMI85.33 | FANHSIDAQTYLLVEVVTDAGFVGL | TCKATBSWGTGGTTBGCRAA |
| Dienelactone hydrolase | ||
| DLH49.82 | SXAPPTPAHISIQQNR | |
| DLH71.46 | XYDNAGHSFLAGLTEPNHPSTAAAH | |
| DLH84.65 | TPGTDPVPYLHASSDTG |
a
Segments used for the design of oligonucleotides for cloning are underlined. Letters in parentheses indicate uncertain amino acids.
b
From reference 63. The published N-terminal sequence had an aspartate in the second position instead of the asparagine shown here.