TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Relevant genotype | Characteristics | Source or reference |
|---|---|---|---|
| Strains | |||
| 285c | rpoD285 | 10 | |
| CG410 | dnaK756 | 34 | |
| RL721 | rpoC3531(His6) zja::kan | Gift of R. Landick | |
| NUT20 | dnaK756/pUHE212-1 pDMI,1 | This laboratory | |
| NUT21 | dnaK756/pUHE211-1 pDMI,1 | This laboratory | |
| NUT26 | dnaK756/phis176 pDMI,1 | pUHE211-1 derivative (this laboratory) | |
| NUT29 | dnaK756/phis163 pDMI,1 | pUHE212-1 derivative (this laboratory) | |
| NUT33 | dnaK756/phis112 pDMI,1 | pUHE212-1 derivative (this laboratory) | |
| NUT34 | dnaK756/phis174 pDMI,1 | pUHE212-1 derivative (this laboratory) | |
| NUT35 | dnaK756/phis182 pDMI,1 | pUHE211-1 derivative (this laboratory) | |
| NUT48 | dnaK756/phis111 pDMI,1 | pUHE211-1 derivative (this laboratory) | |
| NUT49 | dnaK756/phis161 pDMI,1 | pUHE212-1 derivative (this laboratory) | |
| NUT50 | dnaK756/phis162 pDMI,1 | pUHE212-1 derivative (this laboratory) | |
| NUT53 | dnaK756/phis113 pDMI,1 | pUHE211-1 derivative (this laboratory) | |
| Plasmids | |||
| pUHE211-1 | ς32 C-his, Apr | 5 | |
| pUHE212-1 | ς32 N-his, Apr | 5 | |
| pDMI,1 | lacIq Kmr | 5 | |
| pJET40 | dnaK-P1 promoter | Gift of J. Erickson |