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. 2023 Nov 14;133(12):1040–1055. doi: 10.1161/CIRCRESAHA.123.323571

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© 2023 The Authors.

Circulation Research is published on behalf of the American Heart Association, Inc., by Wolters Kluwer Health, Inc. This is an open access article under the terms of the Creative Commons Attribution Non-Commercial-NoDerivs License, which permits use, distribution, and reproduction in any medium, provided that the original work is properly cited, the use is noncommercial, and no modifications or adaptations are made.

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Figure 4. — Pretreatment with S-nitrosoglutathione (GSNO) enhances Ca²⁺ transient response to isoproterenol in CaMKIIδ (Ca²⁺/calmodulin kinase II delta)-C273S cardiomyocytes. Ca²⁺ transients from wild-type (WT) and CaMKIIδ C273S cardiomyocytes from were recorded at 0.5 Hz under control conditions and with 100 nmol/L ISO or with pretreatment of 150 μM GSNO before ISO exposure. Mean Ca²⁺ transient amplitude (A) and decay (B) data in response to ISO with control KRH buffer (white bars; WT: n=28 cells, N=14 hearts; CaMKIIδ-C273S: n=24 cells, N=14 hearts) or when pretreated with GSNO (gray bars; WT: n=18 cells, N=7 hearts; CaMKIIδ-C273S: n=24, N=9 hearts). Representative line-scans from quiescent WT (C) and CaMKIIδ-C273S (D) cardiomyocytes under control conditions and with ISO or with pretreatment of GSNO before ISO exposure. Mean Ca²⁺ spark frequency (E) data are shown for cardiomyocytes in response to ISO with control buffer (white bars; WT: n=31 cells, N=14 hearts; CaMKIIδ-C273S: n=24 cells, N=9 hearts) or when pretreated with GSNO (gray bars; WT: n=16 cells, N=7 hearts; CaMKIIδ-C273S: n=25 cells, N=9 hearts). Percentage of cardiomyocytes exhibiting Ca²⁺ waves in quiescent WT and CaMKIIδ-C273S cardiomyocytes (F). ɸP<0.05 compared with WT ISO, ^aP=0.0002 vs CaMKIIδ-C273S ISO, ^bP<0.0001 vs WT ISO with GSNO pretreatment.