Photoconversion parameters of NPy-Aa-d, NPy-S, and CPy-S. σI, σII, and σIII, respectively denote the uncaging cross-sections of the caged pyranine derivatives extracted from analyzing (i) the time dependence of the rise of the fluorescence emission at 540 nm for solutions of NPy-Aa-d, at 500 nm for the solution of NPy-S, and at 510 nm for the solution of CPy-S under monochromatic illumination at 365 nm; (ii) the light intensity-dependence of the inverse of the associated characteristic time τ for NPy-Ab, NPy-Ad, NPy-S and CPy-S at 365 nm; (iii) the time evolution of the concentrations in caged pyranine derivative and its photoreleased free pyranine derivative measured by capillary electrophoresis (for NPy-Ab) and HPLC (for NPy-Ad, NPy-S and CPy-S) under illumination at 365 nm. T = 293 K.
| Pyranine derivatives | σ I (m2 mol−1) | σ II (m2 mol−1) | σ III (m2 mol−1) |
|---|---|---|---|
| NPy-Aa | 1.6 ± 0.2 | — | — |
| NPy-Ab | 6.8 ± 0.7 | 6.2 ± 0.6 | 8.2 ± 0.8 |
| NPy-Ac | 18.3 ± 2.0 | — | — |
| NPy-Ad | 15.6 ± 1.6 | 38.9 ± 3.9 | 25.2 ± 2.5 |
| NPy-S | 8.4 ± 0.7 | 16.3 ± 1.6 | 6.0 ± 0.6 |
| CPy-S | 560 ± 56 | 462 ± 46 | 695 ± 70 |