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. 2023 Dec 6;19(12):e1011824. doi: 10.1371/journal.ppat.1011824

Fig 4. CD4+ T cell depletion results in a reduction in the levels of intact HIV proviruses and latently infected cells.

Fig 4

Copies of cell-associated viral RNA (A) and DNA (B) levels in purified CD4+ T cells isolated from pooled MNCs were quantified by qPCR. (C) Copies of intact virus per million purified CD4+ T cells isolated from pooled MNCs were measured by quantitative viral outgrowth assay (QVOA). (D) The frequency of intact virus, 5’ defective virus and 3’ defective virus in purified CD4+ T cells isolated from pooled MNCs was measured by the intact proviral DNA assay (IPDA). MNC: mononuclear cell; Intact, intact virus; 5’ def, 5’ defective virus; 3’ def, 3’ defective virus. Anti-CD4 antibody treated animals (n = 6) are shown in red; control animals (n = 6) are shown in black. T and C in the X axis represent Test and Control, respectively. Data are expressed as mean ± SEM. Statistical analyses were performed using unpaired two-sided Mann–Whitney U-tests. Statistical significance was considered when P < 0.05.