Fig. 8. Distinct responses to α-TRBV antibodies versus SAgs.

(A) Purified PBMC were cultured for 7 days without (Unstim) or with plate-bound α-TRBV20-1^PAR antibody or TSST-1 as in Fig. 2 (C and D) and then stained for CD45RA and CCR7 to analyze the resulting memory phenotypes by flow cytometry after gating on TRBV20-1⁺ cells, for both CD4 (top row) and CD8 (bottom row) cells. Representative data for one donor (C025) for each subset and condition (contour plots) and overlays of the α-TRBV20-1^PAR versus TSST-1 conditions (colored dot plots, right) are shown. (B) Summary data of the proportion of each naïve and memory subsets in the indicated conditions for n = 4 donors.