Skip to main content
. 2023 Dec 6;9(49):eadi8076. doi: 10.1126/sciadv.adi8076

Fig. 2. CTDs determine the unique internalization and trafficking properties of each mGluR subtype.

Fig. 2.

(A to D) Top: Schematics of mGluR CTD chimeras. Bottom: Fluorescence images and line scan profiles (from dotted lines) of live cells expressing SNAP-mGluR chimeras (red) and β-arr2–YFP (green) following 30 min of Glu treatment. (E) Quantification of surface fluorescence with and without 1 mM Glu treatment across constructs. (F) Quantification of total fluorescence with and without 1 mM Glu across constructs. (G) Schematics summarizing the main findings. mGluR2-mGluR8CTD internalizes with β-arrs and undergoes degradation, while mGluR8-mGluR2CTD does not recruit β-arrs or internalize. mGluR3-mGluR8CTD shows cointernalization with β-arrs and undergoes degradation, while mGluR8-mGluR3CTD shows cointernalization with β-arrs but recycles to the plasma membrane. Scale bars, 10 μm. Data are represented as means ± SEM. Unpaired t tests, **P < 0.01 and ***P < 0.001.