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. 2023 Dec 6;12:RP89512. doi: 10.7554/eLife.89512

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© 2023, Yamaguchi et al

This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.

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Figure 1. — Viability of myotubes was assessed by (A) MTT assay and (B) Zombie Red staining at 24 hr after US irradiation. (red: Zombie Red, blue: DAPI). (C) Total protein content was measured by the Bradford method at 24 hr after US irradiation. (D) Energy metabolism in C2C12 myotubes was measured by citrate synthase assay at 24 hr after US irradiation. The US intensities of 1.0 W/cm², 2.0 W/cm², and 3.0 W/cm² were tested. Data are expressed as mean ± SEM. n=4.