Table 1.
Primers used for cloning, deletion, site-directed mutagenesis, and ChIP-PCR of ATGL promoter
| Primer name | Primer | Primer sequences2 (5ʹ-3ʹ) | Binding(bp) |
|---|---|---|---|
| Cloning primers | Forward | GGCAGAACTCGCAATCCTA | −2024 |
| Reverse | CACGCCGATATGGTAGAC | +216 | |
| 5ʹ deletion primers | F-2024 F-1399 |
ATTACGCGTGGCAGAACTCGCAATCCTA ATTACGCGTCTCAGTCCTTACTTGAACCT | −2024 −1399 |
| F-882 | ATTACGCGTTCAGAACAGAGACAGGACT | −882 | |
| F-524 | ATTACGCGTGATTCTGTGATGAGTCTCG | −524 | |
| R + 216 | GGAAGATCTCACGCCGATATGGTAGAC | +216 | |
| Site-directed | FKH1 | CGCTGCCTGTGTGTTTACGGGG | −549 |
| ChIP-PCR | FKH –mut | CGCTGCCTGTGCGGATACGGGG | −549 |
| FKH1-anti-mut | CCCCGTATCCGCACAGGCAGCG | −549 | |
| FKH2 | AGACGCCAGTGTTCTCTCTGGGAAT | −585 | |
| FKH 2-mut | AGACGCCAGCGGTATCTCTGGGAAT | −585 | |
| FKH 2-anti-mut | ATTCCCAGAGATACCGCTGGCGTCT | −585 | |
| FKH1-F | GCTGCCTGTGTGTTTACGG | −548 | |
| FKH1-R | CCCAGAGCAGTGCGGAT | −352 | |
| FKH2-F | GTAGACCCACAGCCCAAGGA | −707 | |
| FKH2-R | GTAAACACACAGGCAGCG | −552 |
“+” and “−” represent upstream and downstream from the transcriptional start site; bold represents mutation sites, Italics indicate restriction enzyme sites.