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Population distributions of tryptophan (Trp)–trp RNA-binding attenuation protein (TRAP) complexes reveal homotropic cooperativity in Trp binding to TRAP. The mass spectrum for the 19+ charge state of 1 μM TRAP incubated with the indicated concentration of Trp is displayed (color), overlaid with simulated populations computed from fits to the nearest-neighbor (NN) cooperative model (gray). Figure adapted with permission from Reference 72; copyright 2020 American Chemical Society.
