Fig. 4.

Translation inhibition suppresses pararosaniline toxicity. a) Schematic of targeted RNAi library screen for suppressor of pararosaniline's effect on the in vivo splicing reporter. b) Representative fluorescent images showing effects of candidate gene knockdowns on the splicing reporter after exposure to 200 µM of pararosaniline in liquid media for 24 h. c) Effects of T19A6.4, rpl-3, and rps-28 gene knockdown on C. elegans survival to 0.5–2 mM of pararosaniline on solid media. *P < 0.05, **P < 0.01, and ***P < 0.001 as determined by the log-rank test. Three independent trials were performed with trial 1 shown. Detailed statistics are shown in Supplementary Table 3.