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. 2023 Nov 11;9(1):12–23. doi: 10.1016/j.ncrna.2023.11.006

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© 2023 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Fig. 4 — The effect of HOXD-AS1 overexpression and knockdown on HTR-8/SVneo cells.

A The efficacy of HOXD-AS1 overexpression and knockdown in HTR-8/SVneo cells. B Cell activity detected by CCK-8 assay. C The level of 2 inflammatory factors in the supernatant of HTR-8/SVneo cells detected by ELISA. D Cell apoptosis detected by flow cytometry. E Cell invasion. F Cell migration. G The mRNA level of miR-135a and β-TRCP. H The protein level of β-TRCP and NF-κB pathway. pcDNA4.0, the control of HOXD-AS1 overexpression group; pcDNA4.0-HOXD-AS1, HOXD-AS1 overexpression group; shNC, the control of HOXD-AS1 knockdown group; shHOXD-AS1 #1/2, HOXD-AS1 knockdown via 2 different fragments. ns, not significant; ***p < 0.001, vs. pcDNA4.0 group; ###p < 0.001, vs. shNC group.