TABLE 1.

Effect of rssB on RpoS and lambda O protein degradation

Strain	Genotype	Growth phase	Half-life (min)a
			λ O	RpoS
YN186	clp+ rssB+	Exponential	<2	<2
YN186	clp+ rssB+	Stationary	3	7
YN187	clpP::Cat	Exponential	>30	>30
YN188	rssB::Tet	Exponential	<2	>30
YN189	prssB+/clp+	Exponential	<2	Not detectable

^aGels obtained from experiments shown in Fig. 1 were scanned with an Eagle Eye II scanner and normalized for protein after 8 min of induction, and the half-lives were estimated. While these are results of a single set of experiments, the general pattern was the same for other experiments. In another strain background (MC4100 [4]), we saw similar results for lambda O protein turnover, although RpoS was somewhat more stable during logarithmic growth in our MC4100 derivative.