Table 1.
Host | Isotype | Clonality | Manufacturer | |
---|---|---|---|---|
Primary antibody | ||||
Vimentin | Rabbit | IgG | Monoclonal | CST, Danvers, MA, USA |
HMW | Mouse | IgG | Monoclonal | Thermo Fisher Scientific Inc., Waltham, MA, USA |
α-SMA | Rabbit | IgG | Monoclonal | CST, Danvers, MA, USA |
F-actin | Mouse | IgM | Monoclonal | Abcam, Cambridge, UK |
Fibronectin | Rabbit | IgG | Monoclonal | Abcam, Cambridge, UK |
Vinculin | Rabbit | IgG | Monoclonal | Abcam, Cambridge, UK |
Tenascin C | Rabbit | IgG | Monoclonal | CST, Danvers, MA, USA |
PDGFR-α | Rabbit | IgG | Monoclonal | CST, Danvers, MA, USA |
PDGFR-β | Rabbit | IgG | Monoclonal | CST, Danvers, MA, USA |
FAP | Rabbit | IgG | Polyclonal | Abcam, Cambridge, UK |
PDPN | Mouse | IgG | Monoclonal | Abcam, Cambridge, UK |
TGM2 | Mouse | IgG | Monoclonal | Abcam, Cambridge, UK |
IL-6 | Rabbit | IgG | Monoclonal | CST, Danvers, MA, USA |
pSMAD 2/3 | Rabbit | IgG | Monoclonal | CST, Danvers, MA, USA |
SMAD 2/3 | Rabbit | IgG | Monoclonal | CST, Danvers, MA, USA |
β-catenin | Rabbit | IgG | Monoclonal | CST, Danvers, MA, USA |
pAKT | Rabbit | IgG | Monoclonal | CST, Danvers, MA, USA |
AKT | Rabbit | IgG | Polyclonal | CST, Danvers, MA, USA |
pERK 1/2 | Rabbit | IgG | Polyclonal | CST, Danvers, MA, USA |
ERK 1/2 | Rabbit | IgG | Monoclonal | CST, Danvers, MA, USA |
pRAF | Rabbit | IgG | Monoclonal | Abcam, Cambridge, UK |
RAF | Rabbit | IgG | Polyclonal | Abcam, Cambridge, UK |
HRAS | Rabbit | IgG | Polyclonal | Abcam, Cambridge, UK |
KRAS | Rabbit | IgG | Polyclonal | Abcam, Cambridge, UK |
ROCK1 | Rabbit | IgG | Monoclonal | CST, Danvers, MA, USA |
MLCK | Rabbit | IgG | Polyclonal | Thermo Fisher Scientific Inc., Waltham, MA, USA |
β-actin | Rabbit | IgG | Monoclonal | CST, Danvers, MA, USA |
Secondary antibody | ||||
Anti-rabbit IgG, HRP-linked | Goat | IgG | CST, Danvers, MA, USA | |
Anti-mouse IgG, HRP-linked | Horse | IgG | CST, Danvers, MA, USA |