Table 2.
Reagents used for immunofluorescence
| Host | Isotype | Clonality | Manufacturer | |
|---|---|---|---|---|
| Primary antibody | ||||
| Smooth muscle actin | Mouse | IgG | Monoclonal | Dako, Glostrup, Denmark |
| Vimentin | Mouse | IgG | Monoclonal | Dako, Glostrup, Denmark |
| Fibronectin | Rabbit | IgG | Polyclonal | Dako, Glostrup, Denmark |
| Tenascin C | Rabbit | IgG | Monoclonal | CST, Danvers, MA, USA |
| Secondary antibody | ||||
| Anti-rabbit IgG, Alexa Fluor™ Plus 488 | Goat | IgG | Polyclonal | Invitrogen, Carslbad, CA, USA |
| Anti-mouse IgG, Alexa Fluor™ Plus 488 | Goat | IgG | Polyclonal | Invitrogen, Carslbad, CA, USA |
| Anti-rabbit IgG, Alexa Fluor™ Plus 594 | Goat | IgG | Polyclonal | Invitrogen, Carslbad, CA, USA |
| Anti-mouse IgG, Alexa Fluor™ Plus 594 | Goat | IgG | Polyclonal | Invitrogen, Carslbad, CA, USA |