TABLE 1.
Gene | Forward and reverse primer | PCR product size (bp) | Probe | Reference |
---|---|---|---|---|
sfaA1 | 5′ CGG TGT GCG TAG TTC AAT 3′ | 32 | ||
sfaA2 | 5′ CAC CCG CAT GGA TAA AAA 3′ | 641 | sfa1 | 32 |
sfaG1 | 5′ CAT TAA CTC CCG AAA CTT 3′ | 29 | ||
sfaH2 | 5′ CAT TAC CGC CAC AAC TGC 3′ | 1,862 | sfa2 | 29 |
papA1 | 5′ TTA AAG GTA ATC GGG TCA T 3′ | 22 | ||
papA2 | 5′ GGA ATC AGA GAA AAG GTT 3′ | 856 | pap1 | 22 |
papA1 | 5′ TTA AAG GTA ATC GGG TCA T 3′ | 22 | ||
papC2 | 5′ CGC TTC AGG TCA ACA GAG G 3′ | 3,512 | pap2 | 22 |
papH1 | 5′ AAT ACT GGG GAG AAG AGC AC 3′ | 22 | ||
papF2 | 5′ ATT ACG AAA GGG CAC TGA AG 3′ | 6,086 | pap3 | 22 |
kpsE1 | 5′ AAA AGA CCC GTG TAG AAG C 3′ | 26 | ||
kpsC2 | 5′ CAA AAG GTC AGA GCC AAG T 3′ | 3,852 | kps1 | 26 |
kpsD2 | 5′ CGTTACGGG AAT GCT GCT T 3′ | 26 | ||
kpsM1 | 5′ GTC CAG AAA GTC ACC GTA GA 3′ | X53819a | ||
kpsT2 | 5′ CAA TCG CCA CAT CAC AAA AC 3′ | 1,335 | kps2 | X53819a |
hly1 | 5′ TCA GTC CTC TTT CTT TCC T 3′ | U12572a | ||
hly2 | 5′ CTC TGG CAA CGG TCT CTC C 3′ | 2,899 | hly1 | U12572a |
hlyA1 | 5′ GAC AAA GCA CGA AAG ATG 3′ | 8 | ||
hlyA2 | 5′ CAA CTG CAA TAA AGA AGC 3′ | 2,930 | hly2 | 8 |
GenBank accession number.