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. 2023 Nov 22;4(4):102729. doi: 10.1016/j.xpro.2023.102729

Figure 3.

Figure 3

Using iMbody in ELISA

(A) ELISA represents the binding of 10 nM iMbody to four different iM-forming oligonucleotides and a range of controls.

(B) ELISA shows the binding of serially diluted iMbody to iM-Myc oligonucleotide, which was annealed in the presence of various concentrations of SWNT-COOH or DMF (used to disperse SWNT-COOH). The plotted values represent the mean ± standard error of two replicates, obtained through a serial dilution of iMbody starting from 10 nM to 19.5 pM. Curve fitting was conducted using the "One site - Specific binding" equation in GraphPad Prism. The oligonucleotide sequences are provided in Table 2.