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| Reagent | Final concentration | Amount |
|---|---|---|
| NaH2PO4·H2O | 50 mM | 6.90 g |
| NaCl | 300 mM | 17.54 g |
| Imidazole | 500 mM | 34.04 g |
| ddH2O | N/A | Up to 1 L |
| Total | N/A | 1 L |
Dissolve NaH2PO4, NaCl and imidazole in 600 mL ddH2O. Adjust the pH to 8.0 using 5 M NaOH and 5 M HCl. Bring it to a final volume of 1 L using ddH2O. Filter the solution using a 0.22-μm vacuum filter unit. Can be stored at room temperature (∼22°C) for several months.
