Antibodies |
|
Mouse monoclonal anti-FLAG M2-peroxidase (HRP) antibody (1:15,000 dilution for ELISA) |
Sigma-Aldrich |
Cat# A8592; RRID: AB_439702
|
Rabbit monoclonal anti-FLAG antibody (1:600 dilution for immunostaining) |
Cell Signaling Technology |
Cat# 14793S; RRID: AB_2572291
|
Goat anti-rabbit Alexa Fluor 488 conjugated antibody (1:400 dilution for immunostaining) |
Cell Signaling Technology |
Cat# 4412S; RRID: AB_1904025
|
|
Bacterial and virus strains |
|
BL21(DE3) competent E. coli
|
New England Biolabs |
Cat# C2527H |
|
Chemicals, peptides, and recombinant proteins |
|
Kanamycin sulfate from Streptomyces kanamyceticus
|
Sigma-Aldrich |
Cat# k1377 |
BD Difco dehydrated culture media: LB agar, Miller (Luria-Bertani) |
Fisher Scientific |
Cat# 244520 |
BD Difco dehydrated culture media: LB broth, Miller (Luria-Bertani) |
Fisher Scientific |
Cat# 244620 |
Glycerol |
Sigma-Aldrich |
Cat# G5516 |
Isopropyl β-D-1-thiogalactopyranoside (IPTG) |
Sigma-Aldrich |
Cat# 16758 |
Ni-NTA agarose |
QIAGEN |
Cat# 30210 |
Dulbecco’s phosphate-buffered saline (DPBS) |
Thermo Fisher Scientific |
Cat# 14190144 |
NuPAGE 4%–12%, Bis-Tris, mini protein gels |
Thermo Fisher Scientific |
Cat# NP0322BOX |
NuPAGE MES SDS running buffer, 20× |
Thermo Fisher Scientific |
Cat# NP0002 |
NuPAGE LDS sample buffer, 4× |
Thermo Fisher Scientific |
Cat# NP0007 |
NuPAGE sample reducing agent, 10× |
Thermo Fisher Scientific |
Cat# NP0009 |
PageRuler Plus prestained protein ladder, 10–250 kDa |
Thermo Fisher Scientific |
Cat# 26619 |
EZBlue gel staining reagent |
Sigma-Aldrich |
Cat# G1041 |
NaH2PO4·H2O |
Sigma-Aldrich |
Cat# S9638 |
Sodium chloride (NaCl) |
Ajax Finechem |
Cat# AJA465 |
Sodium hydroxide (NaOH) |
Ajax Finechem |
Cat# AJA482 |
Potassium chloride (KCl) |
Ajax Finechem |
Cat# AJA383 |
Imidazole |
Sigma-Aldrich |
Cat# I2399 |
2-Mercaptoethanol |
Sigma-Aldrich |
Cat# M3148 |
Nuclease-free duplex buffer |
Integrated DNA Technologies |
Cat# 11-05-01-03 |
NeutrAvidin |
Thermo Fisher Scientific |
Cat# 31000 |
Bovine serum albumin (BSA) |
Sigma-Aldrich |
Cat# A9647 |
Tween 20 |
Sigma-Aldrich |
Cat# P9416 |
Tris(hydroxymethyl)aminomethane |
Merck Millipore |
Cat# 108382 |
Carbon nanotube, single-walled, carboxylic acid functionalized (SWNT-COOH) |
Sigma-Aldrich |
Cat# 652490 |
N,N-Dimethylformamide (DMF) |
Sigma-Aldrich |
Cat# D4551 |
UltraPure salmon sperm DNA solution |
Thermo Fisher Scientific |
Cat# 15632011 |
Tetramethylbenzidine (TMB) chromogen solution |
Thermo Fisher Scientific |
Cat# 002023 |
Hydrochloric acid 37% (HCl) |
RCI Labscan |
Cat# RP1107 |
Pure ethanol |
Sigma-Aldrich |
Cat# E7023 |
Dulbecco’s modified Eagle’s medium (DMEM), high glucose, pyruvate |
Thermo Fisher Scientific |
Cat# 11995065 |
Fetal bovine serum (FBS) |
Sigma-Aldrich |
Cat# F9423 |
Penicillin-Streptomycin |
Sigma-Aldrich |
Cat# P4333 |
Poly-L-lysine solution 0.01%, sterile-filtered |
Sigma-Aldrich |
Cat# P4707 |
Trypsin-EDTA solution |
Sigma-Aldrich |
Cat# T3924 |
Pierce 16% formaldehyde (w/v), methanol-free |
Thermo Fisher Scientific |
Cat# 28908 |
Triton X-100 (10% w/v) |
Sigma-Aldrich |
Cat# 11332481001 |
ProLong Gold antifade reagent with DAPI |
Cell Signaling Technology |
Cat# 8961S |
Alexa Fluor 555 conjugated phalloidin (1:20 dilution for immunostaining) |
Cell Signaling Technology |
Cat# 8953S |
|
Experimental models: Cell lines |
|
HeLa cells |
ATCC |
Cat# CCL-2 |
|
Oligonucleotides |
|
T7 promoter forward primer |
Integrated DNA Technologies |
5′-TAATACGACTCACTATAGGG |
T7 terminal reverse primer |
Integrated DNA Technologies |
5′-GCTAGTTATTGCTCAGCGG |
|
Recombinant DNA |
|
pSANG10-3F-iMbody plasmid |
This paper |
Addgene plasmid #184496, www.addgene.org/184496/
|
|
Software and algorithms |
|
BioRender |
BioRender |
https://biorender.com/ |
Prism |
Dotmatics |
https://www.graphpad.com/scientific-software/prism/ |
SnapGene |
Dotmatics |
http://www.snapgene.com |
Fiji |
Schindelin et al.7
|
https://imagej.net/software/fiji/downloads |
|
Other |
|
125 mL Erlenmeyer cell culture flasks with vented cap |
Sigma-Aldrich |
Cat# CLS431143 |
0.2-μm syringe filter |
Sartorius |
Cat# 16534-K |
Disposable cuvettes with 220–1600 nm spectral range |
Eppendorf |
Cat# 0030106300 |
Nalgene centrifuge bottles, style 3120 |
Sigma-Aldrich |
Cat# B1283 |
0.22-μm vacuum filter units |
Sigma-Aldrich |
Cat# 431098 |
Gravity-flow chromatography column |
Bio-Rad |
Cat# 7372522 |
Amicon Ultra-15 centrifugal filter units, 10 kDa |
Millipore |
Cat# UFC901024 |
Costar Spin-X centrifuge tube filters |
Sigma-Aldrich |
Cat# CLS8161 |
96 well MicroWell MaxiSorp flat-bottom plate |
Sigma-Aldrich |
Cat# M9410 |
SealPlate film |
Sigma-Aldrich |
Cat# Z369659 |
TC-treated 12-well plate |
Sigma-Aldrich |
Cat# CLS3513 |
75 cm2 cell culture flask |
Sigma-Aldrich |
Cat# CLS430641U |
Hemocytometer |
Sigma-Aldrich |
Cat# Z359629 |
Miniprep kit |
QIAGEN |
Cat# 27104 |
Shaking incubator (CO2 regulator is not required) |
Eppendorf |
New Brunswick Innova 42R |
Erlenmeyer baffled cell culture flasks |
Sigma-Aldrich |
Cat# CLS431256 |
UV-visible spectrophotometer |
Thermo Fisher Scientific |
NanoDrop One/Oneᶜ |
High-speed refrigerated centrifuge with R10A5 rotor |
Hitachi |
CR22G III |
Vacuum pump and a connecting system |
QIAGEN |
QIAvac system |
Roller |
IKA |
Roller 6 digital |
Electrophoresis power supply |
Bio-Rad |
PowerPac Basic |
Mini gel tank |
Thermo Fisher Scientific |
Cat# A25977 |
Microplate shaker |
Neuation |
iShak Quattro |
Microplate reader |
PerkinElmer |
EnSight |
Tissue culture incubator with CO2 regulator |
Thermo Fisher Scientific |
Heracell Vios 160i CR |
Menzel coverslips, 13 mm, #1.5 |
Fisher Scientific |
Cat# 11588492 |
Universal microscope glass slide, frosted one end two sides, thickness: 1.0–1.2 mm |
Livingstone |
Cat# 7107-U |
Confocal microscope |
Leica Microsystems |
TCS SP8 DLS |