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. 2023 Nov 22;4(4):102729. doi: 10.1016/j.xpro.2023.102729
REAGENT or RESOURCE SOURCE IDENTIFIER
Antibodies

Mouse monoclonal anti-FLAG M2-peroxidase (HRP) antibody (1:15,000 dilution for ELISA) Sigma-Aldrich Cat# A8592; RRID: AB_439702
Rabbit monoclonal anti-FLAG antibody (1:600 dilution for immunostaining) Cell Signaling Technology Cat# 14793S; RRID: AB_2572291
Goat anti-rabbit Alexa Fluor 488 conjugated antibody (1:400 dilution for immunostaining) Cell Signaling Technology Cat# 4412S; RRID: AB_1904025

Bacterial and virus strains

BL21(DE3) competent E. coli New England Biolabs Cat# C2527H

Chemicals, peptides, and recombinant proteins

Kanamycin sulfate from Streptomyces kanamyceticus Sigma-Aldrich Cat# k1377
BD Difco dehydrated culture media: LB agar, Miller (Luria-Bertani) Fisher Scientific Cat# 244520
BD Difco dehydrated culture media: LB broth, Miller (Luria-Bertani) Fisher Scientific Cat# 244620
Glycerol Sigma-Aldrich Cat# G5516
Isopropyl β-D-1-thiogalactopyranoside (IPTG) Sigma-Aldrich Cat# 16758
Ni-NTA agarose QIAGEN Cat# 30210
Dulbecco’s phosphate-buffered saline (DPBS) Thermo Fisher Scientific Cat# 14190144
NuPAGE 4%–12%, Bis-Tris, mini protein gels Thermo Fisher Scientific Cat# NP0322BOX
NuPAGE MES SDS running buffer, 20× Thermo Fisher Scientific Cat# NP0002
NuPAGE LDS sample buffer, 4× Thermo Fisher Scientific Cat# NP0007
NuPAGE sample reducing agent, 10× Thermo Fisher Scientific Cat# NP0009
PageRuler Plus prestained protein ladder, 10–250 kDa Thermo Fisher Scientific Cat# 26619
EZBlue gel staining reagent Sigma-Aldrich Cat# G1041
NaH2PO4·H2O Sigma-Aldrich Cat# S9638
Sodium chloride (NaCl) Ajax Finechem Cat# AJA465
Sodium hydroxide (NaOH) Ajax Finechem Cat# AJA482
Potassium chloride (KCl) Ajax Finechem Cat# AJA383
Imidazole Sigma-Aldrich Cat# I2399
2-Mercaptoethanol Sigma-Aldrich Cat# M3148
Nuclease-free duplex buffer Integrated DNA Technologies Cat# 11-05-01-03
NeutrAvidin Thermo Fisher Scientific Cat# 31000
Bovine serum albumin (BSA) Sigma-Aldrich Cat# A9647
Tween 20 Sigma-Aldrich Cat# P9416
Tris(hydroxymethyl)aminomethane Merck Millipore Cat# 108382
Carbon nanotube, single-walled, carboxylic acid functionalized (SWNT-COOH) Sigma-Aldrich Cat# 652490
N,N-Dimethylformamide (DMF) Sigma-Aldrich Cat# D4551
UltraPure salmon sperm DNA solution Thermo Fisher Scientific Cat# 15632011
Tetramethylbenzidine (TMB) chromogen solution Thermo Fisher Scientific Cat# 002023
Hydrochloric acid 37% (HCl) RCI Labscan Cat# RP1107
Pure ethanol Sigma-Aldrich Cat# E7023
Dulbecco’s modified Eagle’s medium (DMEM), high glucose, pyruvate Thermo Fisher Scientific Cat# 11995065
Fetal bovine serum (FBS) Sigma-Aldrich Cat# F9423
Penicillin-Streptomycin Sigma-Aldrich Cat# P4333
Poly-L-lysine solution 0.01%, sterile-filtered Sigma-Aldrich Cat# P4707
Trypsin-EDTA solution Sigma-Aldrich Cat# T3924
Pierce 16% formaldehyde (w/v), methanol-free Thermo Fisher Scientific Cat# 28908
Triton X-100 (10% w/v) Sigma-Aldrich Cat# 11332481001
ProLong Gold antifade reagent with DAPI Cell Signaling Technology Cat# 8961S
Alexa Fluor 555 conjugated phalloidin (1:20 dilution for immunostaining) Cell Signaling Technology Cat# 8953S

Experimental models: Cell lines

HeLa cells ATCC Cat# CCL-2

Oligonucleotides

T7 promoter forward primer Integrated DNA Technologies 5′-TAATACGACTCACTATAGGG
T7 terminal reverse primer Integrated DNA Technologies 5′-GCTAGTTATTGCTCAGCGG

Recombinant DNA

pSANG10-3F-iMbody plasmid This paper Addgene plasmid #184496, www.addgene.org/184496/

Software and algorithms

BioRender BioRender https://biorender.com/
Prism Dotmatics https://www.graphpad.com/scientific-software/prism/
SnapGene Dotmatics http://www.snapgene.com
Fiji Schindelin et al.7 https://imagej.net/software/fiji/downloads

Other

125 mL Erlenmeyer cell culture flasks with vented cap Sigma-Aldrich Cat# CLS431143
0.2-μm syringe filter Sartorius Cat# 16534-K
Disposable cuvettes with 220–1600 nm spectral range Eppendorf Cat# 0030106300
Nalgene centrifuge bottles, style 3120 Sigma-Aldrich Cat# B1283
0.22-μm vacuum filter units Sigma-Aldrich Cat# 431098
Gravity-flow chromatography column Bio-Rad Cat# 7372522
Amicon Ultra-15 centrifugal filter units, 10 kDa Millipore Cat# UFC901024
Costar Spin-X centrifuge tube filters Sigma-Aldrich Cat# CLS8161
96 well MicroWell MaxiSorp flat-bottom plate Sigma-Aldrich Cat# M9410
SealPlate film Sigma-Aldrich Cat# Z369659
TC-treated 12-well plate Sigma-Aldrich Cat# CLS3513
75 cm2 cell culture flask Sigma-Aldrich Cat# CLS430641U
Hemocytometer Sigma-Aldrich Cat# Z359629
Miniprep kit QIAGEN Cat# 27104
Shaking incubator (CO2 regulator is not required) Eppendorf New Brunswick Innova 42R
Erlenmeyer baffled cell culture flasks Sigma-Aldrich Cat# CLS431256
UV-visible spectrophotometer Thermo Fisher Scientific NanoDrop One/Oneᶜ
High-speed refrigerated centrifuge with R10A5 rotor Hitachi CR22G III
Vacuum pump and a connecting system QIAGEN QIAvac system
Roller IKA Roller 6 digital
Electrophoresis power supply Bio-Rad PowerPac Basic
Mini gel tank Thermo Fisher Scientific Cat# A25977
Microplate shaker Neuation iShak Quattro
Microplate reader PerkinElmer EnSight
Tissue culture incubator with CO2 regulator Thermo Fisher Scientific Heracell Vios 160i CR
Menzel coverslips, 13 mm, #1.5 Fisher Scientific Cat# 11588492
Universal microscope glass slide, frosted one end two sides, thickness: 1.0–1.2 mm Livingstone Cat# 7107-U
Confocal microscope Leica Microsystems TCS SP8 DLS