Fig. 1.

Regulation of PD-L1 by NR4A1/Sp1 in human colon cancer cells. A Model for regulation of PD-L1 and other genes with GC-rich promoters by NR4A1/Sp1 and structure of 3,5-disubstitutedphenyl DIM analogs. SW480 and RKO cells were transfected with oligonucleotides targeting NR4A1 B, PD-L1 C and Sp1 D or treated with mithramycin E and whole cell lysates were analyzed by western blots as outlined in Methods. SW480 and RKO cells F were treated with DIM-3-Br-5-OCF₃ (X = Br, Y = OCF₃, R = H) or DIM-3,5-Cl₂ (X = Y = Cl; R = H) for 24 h and whole cell lysates were analyzed by western blot